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control vector pegfp c1  (TaKaRa)


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    Structured Review

    TaKaRa control vector pegfp c1
    Control Vector Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+control+vector/pm28058752-78-17-21
    Average 86 stars, based on 1 article reviews
    control vector pegfp c1 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Transcriptional Regulation of Pro-apoptotic Protein Kinase C?
    Article Snippet: .. The expression plasmid bearing the cDNA of GFP-PKCδ was a kind gift from Dr. Mary Reyland, University of Colorado Health Sciences Center (Denver), and the pEGFP-C1 control vector was purchased from Clontech. .. The constructs for mammalian expression of pN3-Sp1, pN3-Sp4, and pN3-Sp3 FL encoding both long and short isoforms of Sp3 ( 46 ), the Drosophila actin promoter-driven expression vectors for Sp1 (pPac-Sp1), the short isoforms of Sp3 (pPac-Sp3), the long isoforms of Sp3 (pPac-USp3), the full length of Sp3 (pPac-Sp3 FL, which is equivalent to the mammalian vector pN3-Sp3FL), Sp4 (pPac-Sp4), and β-galactosidase (p97b) ( 47 ), and the “empty” control vectors pN3 and pPac0 were generously provided by Dr. G. Suske (Philipps-Universität Marburg, Germany).

    Article Title: Lysophosphatidic acid stimulates PC-3 prostate cancer cell Matrigel invasion through activation of RhoA and NF-kappaB activity.
    Article Snippet: This study was performed to determine the relationship of lysophosphatidic acid (LPA) stimulation and increased Ras homolog A (RhoA) activity to nuclear factor kappa B (NF-kB) activity, and the role of these factors in regulating prostate cancer cell invasion.. PC-3 high invasive cells demonstrated constitutively increased RhoA, NF-kB, and in vitro Matrigel invasion which were further induced by LPA stimulation or transfection with constitutively active RhoA Q63E mutant.. LPA treatment rapidly and transiently induced RhoA activity followed by maximally increased DNA binding of NF-kB at 1 h and AP-1 at 4 h. The LPA-induced NF-kB DNA binding was preceded by transient IkBa phosphorylation, and decreased total IkBa levels.

    Plasmid Preparation:

    Article Title: Transcriptional Regulation of Pro-apoptotic Protein Kinase C?
    Article Snippet: .. The expression plasmid bearing the cDNA of GFP-PKCδ was a kind gift from Dr. Mary Reyland, University of Colorado Health Sciences Center (Denver), and the pEGFP-C1 control vector was purchased from Clontech. .. The constructs for mammalian expression of pN3-Sp1, pN3-Sp4, and pN3-Sp3 FL encoding both long and short isoforms of Sp3 ( 46 ), the Drosophila actin promoter-driven expression vectors for Sp1 (pPac-Sp1), the short isoforms of Sp3 (pPac-Sp3), the long isoforms of Sp3 (pPac-USp3), the full length of Sp3 (pPac-Sp3 FL, which is equivalent to the mammalian vector pN3-Sp3FL), Sp4 (pPac-Sp4), and β-galactosidase (p97b) ( 47 ), and the “empty” control vectors pN3 and pPac0 were generously provided by Dr. G. Suske (Philipps-Universität Marburg, Germany).

    Article Title: Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues
    Article Snippet: FMNL1’ was subcloned into the pEGFP-C1 vector (Clontech Laboratories; Mountain View, CA) for a GFP tag. .. Cultured cells were transfected with pEGFP-FMNL1’, with an empty pEGFP-C1 control vector (Clontech Laboratories) or pCMV-sport6-FMNL1’ using Lipofectamine 2000 (Invitrogen). ..

    Article Title: Nuclear myosin VI enhances RNA polymerase II-dependent transcription.
    Article Snippet: .. Transient transfections were performed on HeLa cells with GenePORTER transfection reagent (GTS, San Diego, USA), according to the manufacturer’s instructions, using 10 mg of pEGFP-C1 control vector (Clontech) or full-length pEGFP-MyoVI (Aschenbrenner et al., 2003), kindly provided by T. Hasson at UCSD, USA. ..

    Control:

    Article Title: Transcriptional Regulation of Pro-apoptotic Protein Kinase C?
    Article Snippet: .. The expression plasmid bearing the cDNA of GFP-PKCδ was a kind gift from Dr. Mary Reyland, University of Colorado Health Sciences Center (Denver), and the pEGFP-C1 control vector was purchased from Clontech. .. The constructs for mammalian expression of pN3-Sp1, pN3-Sp4, and pN3-Sp3 FL encoding both long and short isoforms of Sp3 ( 46 ), the Drosophila actin promoter-driven expression vectors for Sp1 (pPac-Sp1), the short isoforms of Sp3 (pPac-Sp3), the long isoforms of Sp3 (pPac-USp3), the full length of Sp3 (pPac-Sp3 FL, which is equivalent to the mammalian vector pN3-Sp3FL), Sp4 (pPac-Sp4), and β-galactosidase (p97b) ( 47 ), and the “empty” control vectors pN3 and pPac0 were generously provided by Dr. G. Suske (Philipps-Universität Marburg, Germany).

    Article Title: Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues
    Article Snippet: FMNL1’ was subcloned into the pEGFP-C1 vector (Clontech Laboratories; Mountain View, CA) for a GFP tag. .. Cultured cells were transfected with pEGFP-FMNL1’, with an empty pEGFP-C1 control vector (Clontech Laboratories) or pCMV-sport6-FMNL1’ using Lipofectamine 2000 (Invitrogen). ..

    Article Title: MicroRNA-Mediated Down-Regulation of M-CSF Receptor Contributes to Maturation of Mouse Monocyte-Derived Dendritic Cells
    Article Snippet: The complete ORF of mouse Csf1r was purchased as a full-length cDNA clone (Open Biosystems, IMAGE accession no. 30436119). .. GM-DC (8 × 10 6 cells) were co-electroporated at day 6 of culture with 8 μg of the Csf1r cDNA clone and 2 μg pEGFP-C1 control vector (Clontech) using an Amaxa nucleofector apparatus (Lonza; program Y-01) and Amaxa mouse macrophage nucleofector kit (Lonza) according to the manufacturer’s instructions. .. For control experiments, cells were co-electroporated with 8 μg pCMV-SPORT6 vector and 2 μg pEGFP-C1 vector.

    Article Title: Nuclear myosin VI enhances RNA polymerase II-dependent transcription.
    Article Snippet: .. Transient transfections were performed on HeLa cells with GenePORTER transfection reagent (GTS, San Diego, USA), according to the manufacturer’s instructions, using 10 mg of pEGFP-C1 control vector (Clontech) or full-length pEGFP-MyoVI (Aschenbrenner et al., 2003), kindly provided by T. Hasson at UCSD, USA. ..

    Cell Culture:

    Article Title: Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues
    Article Snippet: FMNL1’ was subcloned into the pEGFP-C1 vector (Clontech Laboratories; Mountain View, CA) for a GFP tag. .. Cultured cells were transfected with pEGFP-FMNL1’, with an empty pEGFP-C1 control vector (Clontech Laboratories) or pCMV-sport6-FMNL1’ using Lipofectamine 2000 (Invitrogen). ..

    Transfection:

    Article Title: Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues
    Article Snippet: FMNL1’ was subcloned into the pEGFP-C1 vector (Clontech Laboratories; Mountain View, CA) for a GFP tag. .. Cultured cells were transfected with pEGFP-FMNL1’, with an empty pEGFP-C1 control vector (Clontech Laboratories) or pCMV-sport6-FMNL1’ using Lipofectamine 2000 (Invitrogen). ..

    Article Title: Nuclear myosin VI enhances RNA polymerase II-dependent transcription.
    Article Snippet: .. Transient transfections were performed on HeLa cells with GenePORTER transfection reagent (GTS, San Diego, USA), according to the manufacturer’s instructions, using 10 mg of pEGFP-C1 control vector (Clontech) or full-length pEGFP-MyoVI (Aschenbrenner et al., 2003), kindly provided by T. Hasson at UCSD, USA. ..

    Stable Transfection:

    Article Title: Lysophosphatidic acid stimulates PC-3 prostate cancer cell Matrigel invasion through activation of RhoA and NF-kappaB activity.
    Article Snippet: This study was performed to determine the relationship of lysophosphatidic acid (LPA) stimulation and increased Ras homolog A (RhoA) activity to nuclear factor kappa B (NF-kB) activity, and the role of these factors in regulating prostate cancer cell invasion.. PC-3 high invasive cells demonstrated constitutively increased RhoA, NF-kB, and in vitro Matrigel invasion which were further induced by LPA stimulation or transfection with constitutively active RhoA Q63E mutant.. LPA treatment rapidly and transiently induced RhoA activity followed by maximally increased DNA binding of NF-kB at 1 h and AP-1 at 4 h. The LPA-induced NF-kB DNA binding was preceded by transient IkBa phosphorylation, and decreased total IkBa levels.



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